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Take the North South Highway starting at the Sungai Besi toll plaza and head South. Exit at Senai / Singapore / Gelang Patah / Tanjung Pelepas toll plaza. Follow signboards stating Singapore / Tanjung Pelepas until you see the PTP exit. Take the exit and 5.4 kms later, you will reach the port. The journey is approximately 380 km and normally takes about 3 ½ hours.

Head North and take the Perling, Singapore (Tuas) exit at Tampoi. Drive pass through 3 traffic lights until you reach Perling toll plaza. Follow signboards stating Tanjung Pelepas / Singapore until you see the exit to PTP. Take the exit and 5.4 kms later, you will reach the port. The whole journey from the center of Johor Bahru takes approximately 45 km and normally takes about 30 minutes.

The best way is to take the Malaysia-Singapore Second Link Highway into Malaysia. After your Immigration and Customs clearance at the Malaysia CIQ Complex (Customs, Immigration & Quarantine) and the toll gates, proceed for approximately 5.4 kms and make your exit at the Tanjung Pelepas turn off.